- 中文名稱
C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)
- 英文名字
- C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 351744
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- This Human CRP ELISA kit is to be used for the in vitro quantitative determination of human C-Reactive Protein (CRP) concentrations in serum. It is intended for research use only and not for use in diagnostic procedures.Principle:This CRP enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for CRP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. CRP, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound CRP and other components of sample.In order to quantitate the amount of CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated antibody specific for CRP is added to each well to "sandwich" the CRP immobilized during the second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of an acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm±2nm. In order to measure the concentration of CRP in the samples, this kit standard (ready-to use) is assayed at the same time as the samples (diluted if necessary with Sample Diluent). This allows the operator to produce a standard curve of Optical Density (O.D.) versus CRP concentration (mg/L). The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: 0.03mg/LDetection Range: 0.005 -0.100mg/LKit Components: 351744A: Microtiter Plate, 1x96wells, Pre-coated with a monoclonal anti-human CRP351744B: CRP Ab (HRP), 1x12ml. Anti-human CRP labeled with HRP351744C: Standard 1, 0mg/L, 1x1ml. Buffered protein base with preservative.351744D: Standard 2, 0.005mg/L, 1x1ml. CRP in buffered protein base with preservative.351744E: Standard 3, 0.010mg/L, 1x1ml. CRP in buffered protein base with preservative.351744F: Standard 4 , 0.025mg/L, 1x1ml. CRP in buffered protein base with preservative.351744G: Standard 5, 0.050mg/L, 1x1ml. CRP in buffered protein base with preservative.351744H: Standard 6, 0.100mg/L, 1x1ml. CRP in buffered protein base with preservative.351744J : TMB Substrate, 1x11ml. Ready to use.351744K: Stop Solution, 1x11ml. 2N HCl. Caution: Caustic Material!351744L: CRP Sample Diluent, 1x50ml. PBS buffer with BSA as preservative.Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Materials Required but not Supplied:1. Single or multi-channel precision pipettes with disposable tips: 5-10ul, 100ul and 500ul 2. Multi-channel pipette reservoir or equivalent reagent container3. Distilled or deionized water4. Test tubes and racks5. Polypropylene tubes or containers (25ml)6. Erlenmeyer flasks: 100ml, 400ml, 1L and 2L7. Microtiter plate reader (450nm±2nm)8. Automatic microtiter plate washer or squirt bottle9. Sodium hypochlorite solution, 5.25% (household liquid bleach)10. Deionized or distilled water11. Plastic plate cover12. Disposable gloves13. Absorbent paperProtocol:1. Add 10ul of Standard or Sample to the appropriate well of the antibody pre-coated Microtiter Plate. 2. Dispense 100ul of conjugate to each well. Thoroughly mix for 30-60 seconds. 3. Cover plate and incubate for 45 minutes at room Ttemperature (18-25°C). 4. Wash the Microtiter Plate five times using 1X Wash Buffer, using one of the specified methods below:Manual Washing: Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well completely with distilled or deionized water, then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure four more times for a total of 5 washes. After final wash, invert plate and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate firmly when washing the plate to assure that all strips remain securely in frame.Automated Washing: Aspirate all wells, then wash plates 5 times using distilled or deionized water. Always adjust your washer to aspirate as much liquid as possible and set fill volume at 350ul/well/wash (range: 350-400ul). After final wash, invert plate and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. It is recommended that the washer be set for a soaking time of 10 seconds or shaking time of 5 seconds between washes.5. Add 100ul TMB Substrate to each well. Cover andiIncubate for 10-15 minutes at room temperature.6. Add 100ul Stop Solution to each well. Mix well.7. Read the Optical Density (O.D.) at 450nm using a microtiter plate reader within 30 minutes.
- 產品特點
- 針對C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1), High Sensitivity BioAssay ELISA Kit (Human)產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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