- 中文名稱
Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)
- 英文名字
- Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)
- 供應商
- USBiological
- 產品貨號
- 351689
- 產品報價
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Intended Use:This Rotavirus Antigen (RV Ag) ELISA Kit is for the qualitative determination of RV Ag in bovine feces. This kit is intended for research use only and may not be used for therapeutic or diagnostic applicationsPrinciple of the Test:This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RV Ag. Samples are pipetted into the wells with Horseradish Peroxidase (HRP)-conjugated antibody. Any virus antigen present in the samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RV Ag bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:351689A: Microtiter Strips, 1x96 wells. Pre-coated.351689B: Negative Control, 1x800ul351689C: Positive Control, 1x00ul351689D: HRP Conjugate, 1x126ml351689E: Wash Buffer, 30X, 1x6mll 351689F: Substrate A, 1x5ml 351689G: Substrate B, 1x5ml351689H: Stop Solution, 1x5ml Storage and Stability:Store kit at 4°C. The unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for up to 2 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Procedure:Bring all reagents and samples to room temperature before use. Centrifuge the samples again after thawing before the assay. It is recommended that all samples be assayed in duplicate.1. Prepare all reagents, and *samples as directed in the previous sections. 2. Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc; store unused wells at 4°C. 3. Set a Blank well (without any solution), three Negative Control wells and two Positive Control wells.4. Add 50ul of Negative Control, Positive Control or Sample per well, then add 50ul of HRP Conjugate to each well except the Blank well. Cover with the provide adhesive strip. Incubate for 15 minutes at room temperature.5. Aspirate each well. Add 50ul Wash Buffer to each well.6. Wash by filling each well with 250ul ddH2O using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher, and let it stand for 30 seconds; complete removal of liquid at each step is essential to good performance. Repeat the process ten times for a toal of ten washes. After the last wash, remove any remaining liquid by aspirating or decanting. Invert the plate and blot it against clean paper towels.7. Add 50ul of Substrate A and 50ul Substrate B to each well. Incubate for 10 minutes at room temperature. Protect from light.8. Add 50ul of Stop Solution to each well; gently tap the plate to ensure thorough mixing.9. Determine the optical density of each well within 10 minutes using a microplate reader set to 450nm. Take blank well as zero.Interpretation of Results:The presence or absence of Rotavirus Antigen is determined by comparing the absorbance of the samples with those of the controls.A Cut-off value is defined as the average Negative Control OD value plus 0.1. If the average Negative Control OD valuel is <0.05, calculate it as 0.05.1. The Negative Control OD value should be less than 0.1 and the Positive Control OD value should be greater than 0.8. Otherwise, the test must be repeated.2. Determination of results:If ODsample is ≥ Cut-off Value: Test is PositiveIf ODsample is < Cut-off Value: Test is Negative If OD sample is close to the Cut-off Value, we recommend repeating the experiment.
- 產品特點
- 針對Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Rotavirus Antigen (RV Ag) BioAssay Qualitative ELISA Kit (Bovine)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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