MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment. Dominant-negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor，which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control and a 35 kDa band can be detected.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 282 of human MyD88
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的MYD88蛋白發生免疫反應.
保存建議
提供的MYD88抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該MYD88抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
