- 中文名稱
LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)
- 英文名字
- LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)
- 供應商
- USBiological
- 產品貨號
- 298428
- 產品報價
- ￥1/384Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.Intended Use:The LSD1 BioAssay Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1. Test Principle:The key to the LSD1 BioAssay Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (lambda ex= 530±13nm, lambda em= 590±18nm) is measured using a plate reader.Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high saltApplications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:Black microtiter plate, 1x 384 wellsLSD1 recombinant enzyme, 1x60ug Methylated peptide substrate (200uM) 1x1ml Amplex Red reagent (10mM) 1x100ul Peroxidase (10U/ml) 1x200ul2x LSD1 assay buffer, 1x20ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)產品后將其置于4°C/-20°C/-70°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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