- 中文名稱
MCP-1, Mouse, BioAssay ELISA Kit (Monocyte Chemoattractant Protein-1)
- 英文名字
- MCP-1, Mouse, BioAssay ELISA Kit (Monocyte Chemoattractant Protein-1)
- 供應商
- USBiological
- 產品貨號
- 216028
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Monocyte chemotactic protein 1(MCP-1), also known as monocyte chemotactic and activating factor(MCAF), lymphocyte-derived chemotactic factor (LDCF) and glioma-derived chemotactic factor (GDF), is a chemotactic cytokine for monocytes.?It is a CC chemokine with two adjacent cystines at its NH2 terminus.?MCP1 is produced by a variety of cell types, including monocytes, lymphocytes, fibroblasts, endothelial cells, epithelial cells and smooth musclecells.?MCP-1 is up-regulated in response to infectious agents, oxidative radicals and pro-inflammatory mediators released by host cells.?The major physiological function of MCP-1 is to mediate host defense. MCP-1 acts as a chemoattractant to recruit monocytes to the infected or injured area and activates the cells to secret cytokines and superoxide, enhances phagocytosis and antigen presentation by macrophages. ?The expression patterns of MCP-1 and IL-8 are similar. Both cytokines will increase upon stimulation with LPS and inflammatory cytokines such as IL-1, TNF-a, and IFN-g.?In mouse fibroblasts, platelet derived growth factor (PDGF) is a strong inducer of MCP-1 mRNA expression but failed to induce IL-8 mRNA, suggesting different regulatory mechanisms. In addition to being chemotactic for monocytes, MCP-1 also activates mouse monocytes to become cytostatic for several mouse tumor cell lines, release lysosomal enzymes, and generate superoxide.Elevated MCP-1 levels are associated with autoimmune disease, allergic inflammation, atherosclerosis, glomerulonephritis, granuloma formation.?This ELISA kit provides a tool for studying MCP-1 expression and its relationship with various diseases in animal model.Intended Use:This Mouse MCP-1 ELISA kit is to be used for the in vitro quantitative determination of mouse Monocyte Chemoattractant Protein-1 (MCP-1) concentrations in serum, cell culture supernatant, and other biological fluids. Sensitivity:<1.7pg/mlRange:7.8-500pg/mlTest Principle:This mouse MCP-1 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse MCP-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse MCP-1, if present, will bind and become immobilized by the antibody pre-coated on the wells. A biotin conjugated antibody specific for mouse MCP-1 is added to each well. The biotin conjugated antibody will bind to the mouse MCP-1 on the plate. The microtiter plate wells are thoroughly washed to remove unbound biotin conjugate and other components of the sample. Avidin has a very high affinity to biotin. In order to quantify the amount of mouse MCP-1 present in the sample, a standardized preparation of avidin conjugated horseradish peroxidase (HRP) is added to each well. Avidin-HRPwill bind to the biotin on plate during incubation. The wells are thoroughly washed to remove all unbound Avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells to which mouse MCP-1, Biotin conjugate and Avidin-HRPare attached will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of mouse MCP-1 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus MCP-1 concentration (pg/ml). The concentration of MCP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:MCP-1 MICROTITER PLATE?BIOTIN CONJUGATE?AVIDIN CONJUGATE?MCP-1 STANDARD??CALIBRATOR DILUENT I?CALIBRATOR DILUENT II?WASH BUFFER (20X/96 wells, 30X/192 wells)??SUBSTRATE A??SUBSTRATE B??STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對MCP-1, Mouse, BioAssay ELISA Kit (Monocyte Chemoattractant Protein-1)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到MCP-1, Mouse, BioAssay ELISA Kit (Monocyte Chemoattractant Protein-1)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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