【干冰】Anti-p19Arf (exon 1b) (RAT) Monoclonal Antibody - 200-501-892-【干冰】Anti-p19Arf (exon 1b) (RAT) Monoclonal Antibody

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背景資料: The Ink4a-Arf locus encodes two tumor suppressor proteins, p16Ink4a and p19Arf.? Expression of Ink4a and Arf is regulated by distinct promoters upstream of alternative first exons whose products are spliced to a common second exon translated in alternative reading frames (from which Arf gets its name). The two genes are induced by different stress signals and can be separately mutated or silenced in tumor cells.? Targeted disruption of either or both genes in mouse leads to spontaneous tumor formation, which is accelerated by exposure of the mutant animals to chemical carcinogens or ionizing radiation.? Inactivation of ARF by mutation, deletion, or epigenetic silencing is observed in many human cancers, underscoring the role of this protein as a potent and ubiquitous tumor suppressor.? p19Arf? is activated by Myc or mutant Ras.? Once activated, p19Arf binds to the p53 negative regulator Mdm2, leading to p53 stabilization and unleashing a p53-dependent transcriptional program that triggers either cell cycle arrest or apoptosis.? p19Arf also has p53-independent inhibitory effects on cell proliferation.? p19Arf is a nucleolar protein and is implicated in ribosomal biogenesis.

免疫原: This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 54-75 of mouse p19Arf protein.

保存建議: Store vial at -20° C prior to opening.? Aliquot contents and freeze at -20° C or below for extended storage.? Avoid cycles of freezing and thawing.? Centrifuge product if not completely clear after standing at room temperature.? This product is stable for several weeks at 4° C as an undiluted liquid.? Dilute only prior to immediate use.

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。