Sheep IgG TrueBlot? is a unique horseradish peroxidase conjugated Anti-Sheep IgG monoclonal secondary antibody. Sheep IgG TrueBlot? enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Sheep IgG TrueBlot?, simply substitute the conventional HRP Anti-Sheep IgG blotting reagent with Sheep IgG TrueBlot? and follow the prescribed protocol for sample preparation and immunoblotting. Sheep IgG TrueBlot? is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of Sheep IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Sheep IgG TrueBlot? with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
