- 中文名稱
PDE1B Cell-Based Reporter BioAssay Kit
- 英文名字
- PDE1B Cell-Based Reporter BioAssay Kit
- 供應商
- USBiological
- 產品貨號
- 171960
- 產品報價
- ￥1/500Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- PDE1B plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5′-monophosphate and prefers cGMP at low substrate levels. The PDE1B Cell-Based Reporter BioAssay Kit is designed for identification of PDE1B inhibitors in cultured cells. The kit includes a recombinant HEK293 cell line that stably expresses human PDE1B, atrial natriuretic peptide (ANP), mammalian expression constructs for ANP receptor A, CNGA2, and aequorin, and buffer for the assay. In the PDE1B reporter assay, PDE1B-HEK293 cells are transiently transfected with expression vectors for atrial natriuretic peptide (ANP) receptor A (ANPRA, a guanylate cyclase), cyclic nucleotide-gated cation channel CNGA2, and the photoprotein aequorin. When cells are activated with ANP, ANP induces the production of cGMP through ANPRA. cGMP can activate the CNGA2 channel and induces Ca2+ influx, resulting in aequorin luminescence. Therefore, the intracellular cGMP level is monitored via aequorin luminescence induced by Ca2+ influx through CNGA2, acting as the intracellular cGMP sensor. PDE1B can reduce the ANP-induced cGMP level in the cells, causing lowered aequorin luminescence. When PDE1B activity is inhibited (for example, by the PDE1 inhibitor BAY607550), then the ANP-induced cGMP level increases, resulting in calcium influx that stimulates luminescence signals. A schematic presentation of this assay is shown in figure 1.Components:PDE1B-HEK293 cell line, 1x1vialANPRA/aequorin Expression vector*, 1x20ug (80ng DNA/ul)CNGA2 Expression vector* 1x20ug (80ng DNA/ul)Atrial natriuretic peptide (ANP), 10um, 1x50ulCoelenterazine, 5mM, 1x50ulCa2+ free assay buffer, 1x100mlBSA (100X), 1x500ulCaCl2, 1M, 1x200ul*The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對PDE1B Cell-Based Reporter BioAssay Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到PDE1B Cell-Based Reporter BioAssay Kit產品后將其置于-20°C/-70°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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