- 中文名稱
Protein A, BioAssay ELISA Kit
- 英文名字
- Protein A, BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- 168614
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Protein A ELISA Kit is a Sandwich ELISA for the highly sensitive detection and/or quantification of Protein A in fluids. The kit is designed to measure trace contamination of Protein A in antibody solutions. The antibodies used in this kit were produced by immunization with recombinant Protein A. Native and recombinant Protein A are quantified in this assay with similar potency.Background:Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sj?holm, 1975).Protein A may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. These conjugates may be used to track immunoglobulins in histochemical, Western Blot and ELISA.Alternatively, Protein A may be immobilized onto a solid support to facilitate the purification and recovery of either polyclonal or monoclonal immunoglobulins. Immobilized protein A has recently been applied therapeutically to treat a variety of humoral diseases (Watson et al, 1989).Protein A, BioAssay ELISA Kit, 96 tests, Quantitative detects samples containing Protein A or purified Ig's contaminated with Protein A.Species Reactivity: ChickenDetection Range: 125-2000pg/mlSample Size: 100ulSensitivity: 50pg/mlAssay Time: 100 minutesKit Components:Anti-Protein A Microwell Strip Plate, 8x12 stripsProtein A Stock, lyophilized Anti-Protein A HRP Conjugate Sample Diluent (20X) Wash Solution (100X) TMB Substrate??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????Sample Recovery:Protein A immunosorbents are widely used for purification of monoclonal and polyclonal antibodies. Possible leakage of Protein A during processing may contaminate the purified antibody preparations. The detection/measurement of contaminating Protein A is complicated by Protein A-IgG binding in the sample, which inhibits the subsequent binding of the Protein A to the ELISA antibodies required for accurate quantification. A sample pretreatment boiling step for Protein A samples containing mouse IgG heat denatures the IgG. This uncouples the Pro A-IgG binding and, thus, allows for accurate Protein A measurement. Appropriate recovery of Protein A in solutions of IgG from other species has not been elucidated with this ELISA. In these cases, the investigator should demonstrate requirements for appropriate Protein A recovery.High and low concentrations of Protein A were mixed into buffer containing mouse IgG at 1mg/ml. Samples were assayed before and after a boiling pretreatment step. Observed assay values in samples containing IgG compared to non-IgG sample values ranged from 41 to 62%. With a boiling pretreatment step, observed IgG sample assay values were 75 to 106% of non-IgG values, indicating accurate quantification of Protein A in the presence of IgG using the pretreatment step.Test Principle:The Protein A ELISA kit is based on the binding of Protein A in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of Protein A present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of Protein A in samples is calculated from a curve of standards containing known concentrations of Protein A.Storage and Stability:The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the label. Stabilities of the working solutions are indicated under Reagent Preparation.Sample Diluent and anti-Protein A-HRP contain Proclin 300 (0.05%, v/v). Stop Solution contains 1% sulfuric acid. Follow good laboratory practices, and avoid ingestion or contact of any reagent with skin, eyes or mucous membranes. All reagents may be disposed of down a drain with copious amounts of water.
- 產品特點
- 針對Protein A, BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Protein A, BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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