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中文名稱

Dihydro-Testosterone BioAssay ELISA Kit

英文名字
Dihydro-Testosterone BioAssay ELISA Kit
供應商
USBiological
產品貨號
167550
產品報價
¥1/96Tests
產品說明書
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背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業??梢詾槟峁┘皶r的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
5a-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity.The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione.The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Intended Use:The Dihydro- Testosterone ELISA kis is for the direct quantitative determination of Dihydrotestosterone by enzyme immunoassay in human serum. For in vitro diagnostic use only.Principle of the Test:The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHT in the sample. A set of standards is used to plot a standard curve from which the amount of DHT in patient samples and controls can be directly read.Detection Range: 0-2500 pg/mlSpecificity: 100%Sensitivity: 6pg/mlKit Components:Microtiter Plate, 1x96wellsDihydrotestosterone-Horseradish Peroxidase (HRP) Conjugate Concentrate, 1x200ulCalibrator A 0pg/ml, 1x2mlCalibrator B 25pg/ml, 1x600ulCalibrator C 100pg/ml, 1x600ulCalibrator D 500pg/ml, 1x600ulCalibrator E 1000pg/ml, 1x600ulCalibrator F 2500pg/ml, 1x600ulControl, 1x600ulWash Buffer Concentrate, 1x50mlAssay Buffer, 1x15mlTMB Substrate, 1x16mlStop Solution, 1x6mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4oC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
產品特點
針對Dihydro-Testosterone BioAssay ELISA Kit該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Dihydro-Testosterone BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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