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中文名稱

Survivin, Human (Surv, Apoptosis Inhibitor Survivin, BIRC5, API4) BioAssay ELISA Kit

英文名字
Survivin, Human (Surv, Apoptosis Inhibitor Survivin, BIRC5, API4) BioAssay ELISA Kit
供應商
USBiological
產品貨號
144046
產品報價
¥1/96Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業??梢詾槟峁┘皶r的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Survivin is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain.The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.Intended Use:Survivin, Human BioAssay ELISA Kit is a sandwich ELISA for quantitative detection of human Surv in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).Range: 62.5-4000pg/mlSensitivity: <2pg/mlSpecificity: Natural and recombinant human SurvCrossreactivity: No detectable crossreactivity with other relevant proteinsKit Components:144046A: Microtiter Plate, 1x96 wells*144046B: Surv Standard, 2x1 vial144046C: Anti-human Surv (Biotin), 1x130ul (1:100 dilution)143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability:Store *144046B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 15-20 minutes.5. Add TMB stop solution and read.
產品特點
針對Survivin, Human (Surv, Apoptosis Inhibitor Survivin, BIRC5, API4) BioAssay ELISA Kit該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Survivin, Human (Surv, Apoptosis Inhibitor Survivin, BIRC5, API4) BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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