LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)-LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)

英文名字: LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)

供應商: USBiological

產品貨號: 137307

產品報價: ￥1/500Tests

產品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: The Wnt / b-catenin signaling pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of b-catenin. b-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.Intended Use:Designed for monitoring the activity of Wnt / b-catenin signaling pathway in the cultured cells.Test Principle:The kit contains transfection- ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non- inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.Kit Components:Reporter (Component A): TCF/LEF luciferase reporter vector + constitutively expressing Renilla luciferase vector. 1x500ul (60ng DNA/ul)Negative Contol Reporter (Component B): Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector. 1x500ul (60ng DNA/ul)Storage and Stability:Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)產品后將其置于-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。