Spirochrome SiR-微管蛋白試劑盒：一鍵解鎖活細胞動態成像

SiR-微管蛋白是基于微管結合藥物多西他賽的。SiR-微管蛋白具有熒光特性、細胞通透性，并且對微管具有很高的特異性。SiR-微管蛋白能夠在不需要基因操作或過表達的情況下對內源性微管進行染色。其遠紅光發射最大限度地減少了光毒性以及樣本的自發熒光。SiR-微管蛋白與綠色熒光蛋白（GFP）和/或 m-櫻桃熒光蛋白兼容?？梢允褂脴藴实?Cy5 濾光片組進行成像。SiR-微管蛋白可用于活細胞和組織的寬場、共聚焦、SIM 或 STED 成像。探針數量允許進行 50 - 200 次染色實驗。

作為Spirochrome在中國的區域總代理，艾美捷科技強烈推薦Spirochrome熱銷產品SiR-微管蛋白試劑盒。產品僅用于科研，不可用于臨床診斷。

SiR-微管蛋白試劑盒

光學性質

λabs 652 nm

εmax 1.0×10⁵ mol^-1·cm^-1

λfl 674 nm

Spirochrome的新產品 SPY650-微管蛋白是 SiR-微管蛋白的改進版本。SPY650-微管蛋白在染色細胞時能夠發出更亮的信號，并且背景更少，同時仍然兼容超分辨率成像。了解更多關于 SPY650-微管蛋白的信息，請點擊這里：SPY650-微管蛋白

*基于以下條件：每次染色實驗使用 0.5 - 1 ml 染色液，探針濃度為 0.5 - 1 uM。通過減少體積或探針濃度，可以進一步增加染色實驗的次數。

最新引用文章

1、Multiscale imaging of cytokinesis

Hümpfer N., Marin Z., ..., Ewers H.

bioRxiv  |  2025 Sep 25  |  Read Article  

"PrePrint: .. cells were seeded onto Geltrex-coated glass-bottom imaging dishes (Zellkontakt, 6160-30).. They were synchronized by a single 2 mM thymidine block for 22 h and released into full medium containing 100 nM SiR-tubulin and 10 μM verapamil (both Spirochrome, SC002) and incubated for 5 h. Prior to live-cell imaging, medium was exchanged to FluoroBrite DMEM (Gibco, A1896701), supplemented with 100 nM SiR-tubulin and 10 μM verapamil.. Ultrastructure Expansion microscopy (U-ExM) of cytokinetic cells.Ultrastructure Expansion microscopy (U-ExM) .." More...

2、Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.

Samuele Ghignoli, Valentina De Lorenzi, ..., Francesco Cardarelli

Traffic (Copenhagen, Denmark)  |  2025 Sep 24  |  40988617  |  Read Article  

".. upkeeping medium and after 15 min of incubation, unless otherwise specified, imaging was performed.. For assessing cytoskeletal components depolymerization, cells were treated for 30 min with 10 μM SiR- Tubulin (Spirochrome, cat# SC002 [62]) to label microtubules or with 10 μM SiR- Actin (Spirochrome, cat# SC001 [62]) to label actin filaments.. For these treatments, 10 μM Nocodazole (Sigma- Aldrich, cat# 487928) and 10 μM Latrunculin- .." More...

3、Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

Hotta Takashi, Pimm Morgan L., ..., Verhey Kristen J.

bioRxiv  |  2025 Sep 23  |  Read Article    |  Bioz AI Brief 

"PrePrint: that lattice state impacts the accessibility of the Y-αCTT. Previous work showed that localization of A1aY1 shifts from cytosolic or plus ends to the microtubule lattice in cultured cells treated with SiR-Tubulin ( Kesarwani et al. 2020 ), a Taxol derivative that can convert GDP-tubulin into a GTP-like expanded state ( Siahaan et al. 2022 ). We thus hypothesized that lattice expansion drives access" More...

Spirochrome 成立于 2015年，總部位于瑞士，是一家專注于活細胞熒光成像技術的生物科技公司。公司核心技術源于國際權威學術期刊《Nature Chemistry》與《Nature Methods》發表的兩項創新研究成果—— SIR與SPY熒光母核。該類染料具有卓越的穩定性、超高熒光信號與極低背景噪聲，能夠突破傳統成像限制，適用于多種超分辨熒光成像技術， 助力科學家開啟活細胞微觀世界的分子尺度探索時代。

作為Spirochrome在中國的區域總代理，艾美捷科技有限公司將為中國客戶提供全面的Spirochrome產品以及客戶訂制化服務，歡迎大家隨時聯系我們。