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牛粒細胞-巨噬細胞集落刺激因子/Bovine GM-CSF重組蛋白中英文說明書

發布者:艾美捷科技    發布時間:2023-07-19     
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艾美捷牛粒細胞-巨噬細胞集落刺激因子/Bovine GM-CSF重組蛋白中文說明書:

目錄編號(規格):

RP0871B-005(5?g)

RP0871B-025(25?g)

RP0871B-100(100?g)

來源:酵母

別名:CSF-2

配方:無載體蛋白冷凍。

重建:用至少含有磷酸鹽的無菌緩沖鹽水重建

0.1%載體蛋白。

穩定性和儲存:在-20°C溫度下,自收到之日起可穩定12個月。在工作等分試樣中儲存時至少可穩定3個月在-20°C下具有載體蛋白。避免反復凍融循環。

分子量:14.3 kDa(計算)

生物活性:重組牛GM-CSF的生物活性為在使用人TF-1的細胞增殖測定中測量細胞系。這種效應的ED50通常為2.5-4.0 ng/mL。

純度:通過SDS-PAGE分析顯示>95%。

純化:離子交換色譜法

Entrez基因ID:281095

氨基酸序列:APTRPNTAT RPWQHVDAIK EALSLLNHSS DTDAVNDTE VVSEKFDSQE ptclctrlkl YKNGLQGSLT SLMGSLTMMA

氨基酸同源性:牛頭(牛)GM-CSF–100%

Bos mutus(野牦牛)GM-CSF–99%

野牛野牛(野牛)GM-CSF–98%

印度博斯(澤布)GM-CSF–98%

原產國:美國

牛粒細胞-巨噬細胞集落刺激因子

牛粒細胞-巨噬細胞集落刺激因子/Bovine GM-CSF重組蛋白英文說明書:

Catalog Number (Quantity): RP0871B-005 (5 ?g)

RP0871B-025 (25 ?g)

RP0871B-100 (100 ?g)

Source: Yeast

Alias: CSF-2

Formulation: Lyophilized without carrier protein.

Reconstitution: Reconstitute with sterile phosphatebuffered saline containing at least

0.1% carrier protein.

?01/18/2019

Stability and Storage: Stable for up to twelve months from date of receipt at -20°C.

Stable for at least 3 months when stored in working aliquots

with a carrier protein at -20°C. Avoid repeated freeze/thaw

cycles.

Molecular Weight: 14.3 kDa (calculated)

Bioactivity: The biological activity of recombinant bovine GM-CSF was

measured in a cell proliferation assay using the human TF-1

cell line. The ED50 for this effect is typically 2.5 - 4.0 ng/mL.

Purity: >95% as visualized by SDS-PAGE analysis.

Purification: Ion-exchange chromatography

Entrez Gene ID: 281095

Amino Acid Sequence: APTRPPNTAT RPWQHVDAIK EALSLLNHSS DTDAVMNDTE

VVSEKFDSQE PTCLQTRLKL YKNGLQGSLT SLMGSLTMMA

THYEKHCPPT PETSCGTQFI SFKNFKEDLK EFLFIIPFDC

WEPAQK (126)

Amino Acid Homology: Bos taurus (cattle) GM-CSF – 100%

Bos mutus (wild yak) GM-CSF – 99%

Bison bison bison (bison) GM-CSF – 98%

Bos indicus (zebu) GM-CSF – 98%

Country of Origin: USA

Warranty: Products are warranted by Kingfisher Biotech, Inc. to meet stated product

specifications and to conform to label descriptions when used, handled and

stored according to instructions. Unless otherwise stated, this warranty is

limited to one year from date of sale or expiry date, whichever comes first.

Kingfisher Biotech’s sole liability for the product is limited to replacement of

the product or refund of the purchase price. Kingfisher Biotech products are

supplied for research applications. They are not intended for medicinal,

diagnostic or therapeutic use.

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