Jena Bioscience/艾美捷6-硫代-GTP：在RNA合成與修飾中的機制研究

Jena Bioscience-6-硫代-GTP的應用：

對大腸桿菌 CTP 合酶的變構作用[1]

抑制 Vac-1-Rac 信號轉導[2]

通過阻斷 GTPase 激活進行免疫抑制[3, 4]

免疫抑制藥物（移植、炎癥）[1, 4]

艾美捷Jena Bioscience 6-硫代-GTP#NU-1106S參數：

適合一般實驗室使用。

運輸：通過凝膠包運輸

儲存條件：儲存于 -20 °C

可以短期暴露于環境溫度（累計最多 1 周）。

保質期：交貨后12個月

分子式： C 10 H 16 N 5 O 13 P 3 S（游離酸）

分子量： 539.24 g/mol（游離酸）

精確質量： 538.97 g/mol（游離酸）

CAS#： 17670-19-8

純度： ≥95%（HPLC）

形式：水溶液

顏色：無色至微黃色

濃度： 10 mM - 11 mM

pH值： 7.5±0.5

光譜性質： λ max  257 nm，ε 8.8 L mmol ^-1  cm ^-1 (Tris-HCl pH 7.5)，λ max  342 nm，ε 24.8 L mmol ^-1  cm ^-1 (Tris-HCl pH 7.5)

Jena Bioscience-6-硫代-GTP文獻參考：

[1] Lunn et al. (2008) Structural requirements for the activation of Escherichia coli CTP synthase by the allosteric effector GTP are stringent, but requirements for inhibition are lax. J. Biol Chem. 283:2010.

[2] Wang et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signaling blockade in a mouse model. Transplant Immunology 18:53.

[3] Poppe et al. (2005) Azothioprine suppresses Ezrin-Radixin-Moesin-dependent T cell-APC conjugation through inhibition of Vav Guanosine exchange activity on Rac proteins. J. Immunology 176:640.

[4] Tiede et al. (2003) CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4+t lymphocytes. J. Clinical Investigation 111:1133.

Karner et al. (2010) Determination of 6-thioguanosine diphosphate and triphosphate and nucleoside diphosphate kinase activity in erythrocytes: novel targets for thiopurine therapy? Ther. Drug Monit. 32 (2):119.

Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.

Du et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signalling blockade in a mouse model. Transplant Immunology 18 (1): 53.

Pestova et al. (2006) Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex. Genes & Development 20:624.

Neurath et al. (2005) 6-Thioguanosine Diphosphate and Triphosphate Levels in Red Blood Cells and Response to Azathioprine Therapy in Crohn's Disease. Clinical Gastroenterology and Hepatology 3 (10): 1007.

Poland et al. (1997) Entrapment of 6-thiophosphoryl-IMP in the active site of crystalline adenylosuccinate synthetase from Escherichia coli. J. Biol. Chem. 272 (24):15200.

Yarbrough et al. (1985) Kinetics of interaction of 2-amino-6-mercapto-9-beta-ribofuranosylpurine 5'-triphosphate with bovine brain tubulin. Biochemistry-US 24 (7):1708.

Fishback et al. (1984) Interaction of 6-mercapto-GTP with bovine brain tubulin - equilibrium aspects. J. Biol. Chem. 259 (3):1968.

Jena Bioscience-6-硫代-GTP相關產品：

CGR-101, Silicone Grease

EN-109, BseCI

NU-1101, N6-Methyl-ATP

NU-1107, Guanosine-3',5'-bisphosphate

NU-1110, 6-Mercaptopurine-riboside-5'-triphosphate

NU-112, 8-Bromo-ADP

NU-118, 8-Bromo-GTP

NU-124, 8-Bromo-GDP

NU-201, Mant-ADP

NU-204, Mant-GDP

JenaBioscience的主要產品包括核苷/核酸制品系列（包括合成的類似物）、重組蛋白及蛋白表達系統、點擊化學產品系列、在結構學研究方面全面提供晶體篩選試劑盒、優化試劑、工具和各類耗材等。此外，還提供分子生物學和生物化學研究的定制試劑與研究服務。艾美捷科技是Jena Bioscience的中國代理商，為科研工作者提供優質的產品與服務。