Biotin-11-dATP：非放射性DNA標記技術的核心

Botin修飾的脫氧腺苷5'-三磷酸被廣泛用于各種非放射性DNA標記反應，包括缺口翻譯、隨機引物標記、cDNA標記和3'-末端標記。生物素化探針已被證明與同源核酸雜交的速度和程度與非生物素化的探針相同。雜交的生物素化DNA探針可以用抗生物素蛋白和鏈霉抗生物素素蛋白檢測。Tris緩沖液（pH 7.5）中的生物素-11-dATP*1 mM*可以通過缺口翻譯、隨機引物、3'-末端標記或PCR過程酶促摻入DNA。數字“11”是指dATP和生物素之間連接體骨架中的碳原子數。生物素和抗生物素蛋白之間更有效的相互作用將發生在較長的連接體上，而較短的連接體將導致dATP更有效地摻入DNA。建議接頭'11'長度對于大多數應用來說是最佳的。Tris緩沖液（pH 7.5）中的生物素-11-dATP*1 mM*在各種雜交應用中產生生物素化的DNA探針，包括Southern印跡、Northern印跡、斑點印跡、固定細胞和組織。其化學性質與PerkinElmer（PE）的NEL540001EA相當。

艾美捷Biotin-11-dATP：

貨號：AAT-17014

規格：25納摩爾

危險性說明短語：H303, H313, H333

危險符號：XN

預期用途：僅限研究使用（RUO）

風險說明短語：R20, R21, R22

儲存：

冷凍（<-15°C）；盡量減少光照暴露

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