PRODUCT CENTER
特色產品純化的蛋白A,適用于ELISA、Western Blot、免疫沉淀和純化實驗。
作為SouthernBiotech在中國的區域總代理,艾美捷科技強烈推薦SouthernBiotech熱銷產品蛋白質A-無偶聯。產品僅用于科研,不可用于臨床診斷。
| 產品名稱 | 貨號 | 詳情 |
蛋白質A-無偶聯 | 7300-01S | 查看 |
蛋白質A-無偶聯:
特異性:與人IgG1、IgG2和IgG4的Fc段結合,也與小鼠IgG2a和IgG2b結合;此外,還與兔、豬、豚鼠和犬的IgG結合。
來源:金黃色葡萄球菌
偶聯物:UNLB(未偶聯)
緩沖液配方:從蒸餾水中凍干
推薦儲存條件:-20°C
操作和儲存:純化的(UNLB)蛋白A以5.0 mg或1.0 mg的純化凍干蛋白形式提供。在復溶前,材料可在-20°C下儲存長達一年。在PBS中復溶至盡可能高的濃度。復溶后,分裝并儲存于-20°C,最長可達六個月。
辣根過氧化物酶(HRP)偶聯物以1.0 mL的形式提供,其母液為50%甘油/50% PBS,pH 7.4。未添加防腐劑??稍?-8°C下儲存,或長期儲存于-20°C。
如果按照指示儲存,試劑在標簽上標明的有效期內是穩定的。
應用:
ELISA – 經質量檢測 1-7
純化
Western Blot 8
免疫沉淀
文獻參考:
1. Sondgeroth K, Blitvich B, Blair C, Terwee J, Junge R, Sauther M, et al. Assessing flavivirus, lentivirus, and herpesvirus exposure in free-ranging ringtailed lemurs in southwestern Madagascar. J Wildl Dis. 2007;43:40-7. (ELISA)
2. Wycoff KL, Belle A, Deppe D, Schaefer L, Maclean JM, Haase S, et al. Recombinant anthrax toxin receptor-Fc fusion proteins produced in plants
protect rabbits against inhalational anthrax. Antimicrob Agents Chemother. 2011;55:132-9. (ELISA)
3. Duthie MS, Truman RW, Goto W, O'Donnell J, Hay MN, Spencer JS, et al. Insight toward early diagnosis of leprosy through analysis of the developing
antibody responses of Mycobacterium leprae-infected armadillos. Clin Vaccine Immunol. 2011;18:254-9. (ELISA)
4. Arzola L, Chen J, Rattanaporn K, Maclean JM, McDonald KA. Transient co-expression of post-transcriptional gene silencing suppressors for
increased in planta expression of a recombinant anthrax receptor fusion protein. Int J Mol Sci. 2011;12:4975-90. (ELISA)
5. Karuppanan K, Duhra-Gill S, Kailemia MJ, Phu ML, Lebrilla CB, Dandekar AM, et al. Expression, purification, and biophysical characterization of a
secreted anthrax decoy fusion protein in Nicotiana benthamiana. Int J Mol Sci. 2017;18:89. (ELISA)
6. Xiong Y, Li Q, Kailemia MJ, Lebrilla CB, Nandi S, McDonald KA. Glycoform modification of secreted recombinant glycoproteins through kifunensine
addition during transient vacuum agroinfiltration. Int J Mol Sci. 2018;19:890. (ELISA)
7. Sukenik SC, Karuppanan K, Li Q, Lebrilla CB, Nandi S, McDonald KA. Transient recombinant protein production in glycoengineered Nicotiana
benthamiana cell suspension culture. Int J Mol Sci. 2018;19:1205. (ELISA)
8. Naskar P, Puri N. Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis. Biol Open. 2017;6:1257-69.(WB)
SouthernBiotech(SBA)公司建于1982年,致力于為免疫研究的科學家提供各種親和純化的多克隆和單克隆二抗和其它特異性抗體,其中包括:熒光素和酶標記物,低內毒素/不含疊氮化物制劑,F(ab)和F(ab’)299片段化抗體,此外還有純化的免疫球蛋白、血清以及細胞外基質蛋白等產品。作為SouthernBiotech在中國的區域總代理,艾美捷科技有限公司將為中國客戶提供全面的SouthernBiotech產品以及客戶訂制化服務。


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