Nature同款，5hmC-羥化酶TET活性/抑制分析試劑盒

DNA胞嘧啶的表觀遺傳修飾通過調節基因轉錄在發育中起關鍵作用。目前研究最多的胞嘧啶修飾是5-甲基胞嘧啶（5mC），它與基因表達的抑制有關。在脫甲基化過程中，5mC氧化為連續的中間狀態，包括5-羥甲基胞嘧啶（5hmC），5-甲?；奏ぃ?fC）和5-羧基胞嘧啶（5caC）。在過去的十年中，研究表明，氧化形式的5mC，尤其是5hmC，除了在活性脫甲基過程中充當“中間體”外，還可能具有多種獨立的功能。很多研究表明，5hmC 表達水 平與基因表達調控、多能干細胞分化、神經元發育、以及腫瘤發生都密切相關。

而參與其中甲基化循環的一個重要酶類，就是TET家族蛋白！人TET蛋白家族包含TET1、TET2和TET3這3個成員，所有TET蛋白都包含一個保守的雙鏈β-螺旋結構域、一個富含半胱氨酸區和C端的核心催化結構域。TET1主要在胚胎干細胞中表達，TET2主要在造血系統中表達，TET3則在 小腦、皮質和海馬體等神經系統中廣泛表達。結果表明，基因組5hmC 水平與 TET 羥化酶活性相關。

那么如何檢測5hmc羥化酶TET的活性呢？

作為專業的生命科學解決方案供應商，艾美捷為您推薦Nature同款方案：

Nature文章結果：

DOI https://doi.org/10.1038/s41590-020-0764-8

1.TET酶活性分析使用P-3086; c. 使用艾美捷推薦的P-1032檢測整體5hmc水平

更多客戶的選擇：

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