Jena Bioscience：脂質立方相（LCP）結晶

脂質立方相（LCP）結晶已經發展成為膜蛋白結晶的一種重要方法，其中脂質單油酸甘油酯（Monoolein）是創建穩定LCP的首選。然而，在過去幾年中，短鏈脂質7.7 MAG^[4,7]、7.8 MAG^[6-8]和7.9 MAG^[5,7]變得越來越受歡迎。最近在將高黏度脂質立方相應用于連續飛秒晶體學（LCP-SFX）方面的進展^[1-3]，通過以下方式進一步加速了LCP方法的成功：

• 將載有晶體的黏性LCP直接送入X射線自由電子激光（XFEL）光束中（與液體注射器相比，減少了樣品消耗)；

• 利用在LCP中生長的固有小晶體；

• 避免了從LCP中進行棘手的晶體裝載。

JBScreen LCP旨在高效篩選LCP中的結晶條件。上述表格中的每種LCP脂質都能創建一個穩定的脂質立方相，可用于與JBScreen LCP進行結晶設置。其96種條件是通過對192種成功通過in meso方法結晶并產生結構的整合膜蛋白進行數據挖掘得出的^[7]。該篩選按沉淀劑的濃度和類型排序，并且不含乙酸鎘。

文獻：

[1] Nogly et al. (2016) Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nat. Commun. 7:12314.

[2] Zhu et al. (2016) Serial Femtosecond Crystallography of Membrane Proteins. Adv. Exp. Med. Biol. 922:151.

[3] Batyuk et al. (2016) Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Sci. Adv. 2:e1600292.

[4] Coincon et al. (2016) Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters. Nat. Struct. Mol. Biol. 23:248.

[5] Li et al. (2015) Ternary structure reveals mechanism of a membrane diacylglycerol kinase. Nat. Commun. 6:10140.

[6] Fowler et al. (2015) Gating Topology of the Proton-Coupled Oligopeptide Symporters. Structure 23:290.

[7] Caffrey (2015) A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Acta Cryst F 71:3.

[8] Li et al. (2013) Crystal structure of the integral membrane diacylglycerol kinase. Nature 497:521.

[9] Misquitta et al. (2004) Rational design of lipid for membrane protein crystallization. Journal of Structural Biology 148:169.